- 1). On a level surface, place the tray with the prepared gel horizontally in the electrophoresis chamber. Pour the electrophoresis buffer over the gel until fully covered. Then, use a pipette to load the first well with bromophenol blue dye. This wells' lane will be known as the marker lane.
- 2). Mix your sample with the loading buffer. Make sure that the mixture is relatively thick to ensure that the sample sinks to the bottom of the gel's buffering solution. Using a pipette, put a tiny drop into the rest of the wells on the gel.
- 3). Place the lid on the chamber and turn on your power source. Tiny bubbles in the buffering solution should be visible to indicate a proper current flow.
- 4). Allow the current to run until the dye in the marker lane is about 2 cm away from the edge of the gel and then turn off the power source.
- 5). Soak the gel in ethidium bromide for approximately 8 minutes. Then, examine the results under an ultraviolet light source. You can use the distance of the bands to perform calculations pertaining to molecular weights or other such information you wish to find.
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